S7901

Sigma

 

Stem Cell Factor human

recombinant, expressed in Escherichia coli, powder, cell culture tested

Synonym:c-Kit ligand, SCF
MDL number:MFCD00165757

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Description

Analysis NoteThe proliferative activity is tested in culture using TF-1 cells or by the dose dependent proliferation of human MO-7e cells.
Physical formLyophilized from a 0.2μm filtered solution in PBS containg 50 μgBSA /μg cytokine,
Biochem/physiol ActionsStem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. SCF is secreted by endothelial cells, fibroblasts, and bone marrow stromal cells as a membrane-bound form which may be cleaved to release the soluble form. Both forms are active in promoting colony formation from murine bone marrow cells, but membrane-bound SCF is more effective in promoting hematopoieses in vivo, suggesting a role in cellular interactions between hematopoietic and stromal cells. The soluble is thought to exist in solution as a noncovalently linked dimer. SCF is structurally related to M-CSF (CSF-1) and Flt-3/Flk-2 Ligand (FL) with all three sharing a similar size, existence of transmembrane and soluble forms, four conserved cysteines, and alternative splicing exon locations, but they share little sequence homology. SCF alone is a modest colony stimulating factor. However, in the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors.

Properties

recombinantexpressed in Escherichia coli
assay>95% (SDS-PAGE)
formpowder
potency2.5-10 ng/mL ED50/EC50
mol wtprotein mol wt 18.5 kDa
total impurities Endotoxin, tested
suitabilitycell culture tested
storage temp.−20°C

Safety

Personal Protective EquipmentEyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
WGK Germany3

References

referenceAshman, L.,, The biology of stem cell factor and its receptor c-Kit. Int. J. Biochem. Cell Biol. 31, 1037-1051, (1999) Abstract
 Linnekin, D., Early signaling pathways activated by c-Kit in hematopoietic cells. Int. J. Biochem. Cell Biol. 31, 1053-1074, (1999) Abstract
 Molineux, G., and McNiece, I., Thomson, A.W., ed., Stem cell factor. The Cytokine Handbook 3rd ed., San Diego, CA , (1998), 713-725
 McNiece, I., and Shieh, J.-H., Nicola, N., ed. Guidebook to Cytokines and Their Receptors New York, NY , (1994), 177-180